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Normally bosses don't spawn, but a couple do respawn when you save and exit the game then reload that save file

Some bosses such as Mothrakk, Skagzilla and others will respawn every time you load the game once you've completed their mission.

Some others include: Sledge, the Rakk Hive, Nine Toes, and Bone Head. Unfortunatly the Vault Boss does not respawn.

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