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Some bosses respawn and other do not. The last boss for example will not respawn in your own Playthrough 2 game, however you can play him again in someone else's game. Bosses that do respawn inlclude Skagzilla, Mothrakk, Bonehead, Taylor Kobb, One Eyed Jack, etc. However, the majority of these bosses require for you to complete their mission before you attempt to fight them again.

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